Capillary action will draw the fluid into the chamber. Loading the Hemacytometer: You can thus multiply the average number of sperm over each central counting area by 10, to obtain the number of sperm per ml of diluted sample. When counting, count only those cells on the lines of two sides of the large square to avoid counting cells twice.
Count sperm heads in the 5 large squares as indicated by the green color. It is important not to overload the chamber, as doing so will give an inaccurate count. You calculating sperm concentrations thus multiply the average calculating sperm concentrations of sperm over each central counting area by 10, to obtain the number of sperm per ml of diluted sample. The example at right shows red lines where cells on the line would be counted. The full grid on a hemacytometer contains nine squares, each of which is 1 mm square see figure below.
Send comments via form or email to rbowen colostate. Be sure you count 5 squares on each side of the hemocytometer and then use the average for your calculations. If red dots represent cells, one calculating sperm concentrations count 3 cells in the top middle large square. The central counting area of the hemacytometer as it will be called here contains 25 large squares and each large square has 16 smaller squares.
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